Elucidating mechanism cellular uptake removal
We concluded that MRC NPs uptake in breast cancer cells is more effective than in normal cells with regard to both the amount of internalized nanomaterial and the achievement of more strategic intracellular distribution.
Overall, our study demonstrates that cellular response after exposure to MRC NPs varies among cell lines and that different basal expression levels of clathrin in cells can define the biological pathway of MRC NPs and their uptake efficacy.
This work evaluates the uptake mechanism of MRC NPs in metastatic and nonmetastatic breast cancer-cell models, comparing them to a nontumor cell line.
MRC NPs uptake in breast cancer cells was more effective than in normal cells, with regard to both the amount of internalized material and the achievement of more strategic intracellular distribution.
Accepted for publication 28 June 2017 Published 2 August 2017 Volume 20 Pages 5511—5523 DOI https://doi.org/10.2147/IJN.
However, the interaction of these NPs with cells, their uptake mechanism, and subcellular localization need to be elucidated.
The development was made with Amersham ECL prime Western blotting detection reagent, and image acquisition and analyses were performed with an Image Quant LAS 4000 (GE Healthcare, Little Chalfont, UK).